› 6xkx

Electron Microscopy
6.1Å resolution

R. capsulatus CIII2CIV tripartite super-complex, conformation A (SC-1A)

Released:
DOI: 10.2210/pdb6xkx/pdb
PDB entry 6xkx coloured by chain, front view.
PDB entry 6xkx coloured by chain, side view.
PDB entry 6xkx coloured by chain, top view.
Source organism: Rhodobacter capsulatus SB 1003
Primary publication:
Cryo-EM structures of engineered active bc1-cbb3 type CIII2CIV super-complexes and electronic communication between the complexes.
Steimle S, van Eeuwen T, Ozturk Y, Kim HJ, Braitbard M, Selamoglu N, Garcia BA, Schneidman-Duhovny D, Murakami K, Daldal F
OpenAccess logo Nat Commun 12 929 (2021)
PMID: 33568648
Related structures: EMD-22228

Function and Biology Details

Reactions catalysed: 
Quinol + 2 ferricytochrome c(Side 2) = quinone + 2 ferrocytochrome c(Side 2) + 2 H(+)(Side 2)
4 ferrocytochrome c + O(2) + 8 H(+)(Side 1) = 4 ferricytochrome c + 4 H(2)O(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-112662 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Ubiquinol-cytochrome c reductase iron-sulfur subunit Chains: E, R
Molecule details ›
Chains: E, R
Length: 191 amino acids
Theoretical weight: 20.47 KDa
Source organism: Rhodobacter capsulatus SB 1003
Expression system: Rhodobacter capsulatus SB 1003
UniProt:
  • Canonical: D5ANZ2 (Residues: 1-191; Coverage: 100%)
Gene names: RCAP_rcc02768, fbcF, petA
Sequence domains:
Cytochrome b Chains: C, P
Molecule details ›
Chains: C, P
Length: 437 amino acids
Theoretical weight: 49.39 KDa
Source organism: Rhodobacter capsulatus SB 1003
Expression system: Rhodobacter capsulatus SB 1003
UniProt:
  • Canonical: D5ANZ3 (Residues: 1-437; Coverage: 100%)
Gene names: RCAP_rcc02769, cytB, petB
Sequence domains:
Cytochrome c1 Chains: D, Q
Molecule details ›
Chains: D, Q
Length: 279 amino acids
Theoretical weight: 30.35 KDa
Source organism: Rhodobacter capsulatus SB 1003
Expression system: Rhodobacter capsulatus SB 1003
UniProt:
  • Canonical: D5ANZ4 (Residues: 1-279; Coverage: 100%)
Gene names: RCAP_rcc02770, petC
Sequence domains: Cytochrome C1 family
cytochrome-c oxidase Chain: n
Molecule details ›
Chain: n
Length: 532 amino acids
Theoretical weight: 58.98 KDa
Source organism: Rhodobacter capsulatus SB 1003
UniProt:
  • Canonical: D5ARP4 (Residues: 1-532; Coverage: 100%)
Gene names: RCAP_rcc01157, ccoN
Sequence domains: Cytochrome C and Quinol oxidase polypeptide I
Cytochrome c domain-containing protein Chain: o
Molecule details ›
Chain: o
Length: 242 amino acids
Theoretical weight: 27 KDa
Source organism: Rhodobacter capsulatus SB 1003
UniProt:
  • Canonical: D5ARP5 (Residues: 1-242; Coverage: 100%)
Gene names: RCAP_rcc01158, ccoO
Sequence domains: Cytochrome C oxidase, mono-heme subunit/FixO
Cbb3-type cytochrome c oxidase subunit CcoP; Cytochrome c-type cyt cy Chain: p
Molecule details ›
Chain: p
Length: 474 amino acids
Theoretical weight: 50.04 KDa
Source organism: Rhodobacter capsulatus SB 1003
Expression system: Rhodobacter capsulatus SB 1003
UniProt:
  • Canonical: D5ARP7 (Residues: 1-297; Coverage: 100%)
  • Canonical: Q05389 (Residues: 31-199; Coverage: 85%)
Gene names: RCAP_rcc01160, RCAP_rcc02501, ccoP, cycY
Sequence domains:

Ligands and Environments


Cofactor: Ligand HEC 11 x HEC
2 bound ligands:
Ligand FES 2 x FES
Ligand CU 1 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.1Å
Relevant EMDB volumes: EMD-22228
Expression system: Rhodobacter capsulatus SB 1003

Quick links

Citations

1 review citation

An evolving view of complex II-noncanonical complexes, megacomplexes, respiration, signaling, and beyond.
Iverson et al. (2023)
5 other articles

1 mention without citation

Structure and Mechanism of Respiratory III-IV Supercomplexes in Bioenergetic Membranes.
Brzezinski et al. (2021)