› 6xov

Electron Microscopy
3.3Å resolution

CryoEM structure of human presequence protease in partial closed state 1

Released:
DOI: 10.2210/pdb6xov/pdb
PDB entry 6xov coloured by chain, front view.
PDB entry 6xov coloured by chain, side view.
PDB entry 6xov coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the mechanisms of human presequence protease conformational switch and substrate recognition.
Liang WG, Wijaya J, Wei H, Noble AJ, Mancl JM, Mo S, Lee D, Lin King JV, Pan M, Liu C, Koehler CM, Zhao M, Potter CS, Carragher B, Li S, Tang WJ
OpenAccess logo Nat Commun 13 1833 (2022)
PMID: 35383169
Related structures: EMD-22281

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-272311 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Presequence protease, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 1014 amino acids
Theoretical weight: 114.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q5JRX3 (Residues: 33-1037; Coverage: 97%)
  • Best match: Q5JRX3-2 (Residues: 33-1038)
Gene names: KIAA1104, MP1, PITRM1, PREP
Sequence domains:
Amyloid-beta protein 40 Chain: B
Molecule details ›
Chain: B
Length: 40 amino acids
Theoretical weight: 4.34 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P05067 (Residues: 672-711; Coverage: 5%)
Gene names: A4, AD1, APP
Sequence domains: Beta-amyloid peptide (beta-APP)
Amyloid-beta precursor protein Chain: C
Molecule details ›
Chain: C
Length: 3 amino acids
Theoretical weight: 273 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-22281
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided

Quick links

Citations

1 citations in other articles

Insulin-Degrading Enzyme Interacts with Mitochondrial Ribosomes and Respiratory Chain Proteins.
Yilmaz et al. (2023)

1 mention without citation

Structural basis for the mechanisms of human presequence protease conformational switch and substrate recognition.
Liang et al. (2022)