› 6xp5

Electron Microscopy
4.2Å resolution

Head-Middle module of Mediator

Released:
DOI: 10.2210/pdb6xp5/pdb
PDB entry 6xp5 coloured by chain, front view.
PDB entry 6xp5 coloured by chain, side view.
PDB entry 6xp5 coloured by chain, top view.
Source organisms:
Primary publication:
Mediator structure and conformation change.
Zhang H, Chen DH, Mattoo RUH, Bushnell DA, Wang Y, Yuan C, Wang L, Wang C, Davis RE, Nie Y, Kornberg RD
Mol Cell 81 1781-1788.e4 (2021)
PMID: 33571424
Related structures: EMD-22284

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
PDBe Complex ID:
PDB-CPX-271200 (preferred)
Entry contents:
15 distinct polypeptide molecules
Macromolecules (15 distinct):
Mediator of RNA polymerase II transcription subunit 21 Chain: U
Molecule details ›
Chain: U
Length: 135 amino acids
Theoretical weight: 15.72 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Chaetomium thermophilum
UniProt:
  • Canonical: G0S8E0 (Residues: 3-137; Coverage: 99%)
Gene name: CTHT_0029550
Sequence domains: Subunit 21 of Mediator complex
Mediator of RNA polymerase II transcription subunit 4 Chain: D
Molecule details ›
Chain: D
Length: 341 amino acids
Theoretical weight: 37.92 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Chaetomium thermophilum
UniProt:
  • Canonical: G0S7Z4 (Residues: 1-341; Coverage: 100%)
Gene names: CTHT_0037540, MED4
Sequence domains: Vitamin-D-receptor interacting Mediator subunit 4
Mediator of RNA polymerase II transcription subunit 6 Chain: F
Mediator of RNA polymerase II transcription subunit 7 Chain: G
Mediator of RNA polymerase II transcription subunit 9 Chain: I
Mediator of RNA polymerase II transcription subunit 14 Chain: N
Mediator of RNA polymerase II transcription subunit 17 Chain: Q
Mediator of RNA polymerase II transcription subunit 31 Chain: Z
Mediator of RNA polymerase II transcription subunit 8 Chain: H
Mediator of RNA polymerase II transcription subunit 11 Chain: K
Mediator of RNA polymerase II transcription subunit 18 Chain: R
Med22 Chain: V
Mediator of RNA polymerase II transcription subunit 1 Chain: a
Unknown peptide Chain: b
HEAT Chain: c

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-22284
Expression system: Chaetomium thermophilum

Quick links

Citations

3 review citations

The Mediator complex as a master regulator of transcription by RNA polymerase II.
Richter et al. (2022)
2 more

1 mention without citation

Assembly of RNA polymerase II transcription initiation complexes.
Farnung et al. (2022)