› 6xrw

X-ray diffraction
1.77Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191481 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Type II toxin-antitoxin system RelE/ParE family toxin Chains: A, C
Molecule details ›
Chains: A, C
Length: 97 amino acids
Theoretical weight: 10.89 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I708 (Residues: 1-93; Coverage: 100%)
Gene name: PA0124
Sequence domains: ParE toxin of type II toxin-antitoxin system, parDE
Ribbon-helix-helix protein CopG domain-containing protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 79 amino acids
Theoretical weight: 8.99 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I707 (Residues: 1-75; Coverage: 100%)
Gene name: PA0125
Sequence domains: Ribbon-helix-helix protein, copG family

Ligands and Environments

3 bound ligands:
Ligand GOL 4 x GOL
Ligand 2PE 1 x 2PE
Ligand BEN 1 x BEN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2221
Unit cell:
a: 98.401Å b: 121.323Å c: 58.584Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.178 0.219
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

IGF-dependent dynamic modulation of a protease cleavage site in the intrinsically disordered linker domain of human IGFBP2.
Jaipuria et al. (2022)
2 more

2 mentions without citation

ParD Antitoxin Hotspot Alters a Disorder-to-Order Transition upon Binding to Its Cognate ParE Toxin, Lessening Its Interaction Affinity and Increasing Its Protease Degradation Kinetics.
Snead et al. (2022)
1 more