› 6xsx

X-ray diffraction
2.1Å resolution

The structure of the catalytic module of the metalloprotease ZmpA from Clostridium perfringens

Released:
DOI: 10.2210/pdb6xsx/pdb
PDB entry 6xsx coloured by chain, front view.
PDB entry 6xsx coloured by chain, side view.
PDB entry 6xsx coloured by chain, top view.
Source organism: Clostridium perfringens ATCC 13124
Primary publication:
Molecular insights into architecturally complex glycopeptidases
Pluvinage B, Ficko-Blean E, Noach I, Stuart C, Thompson N, McClure H, Buenbrazo N, Wakarchuck W, Boraston AB
Proc. Natl. Acad. Sci. U.S.A. (2021)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-268641 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Discoidin domain protein Chain: A
Molecule details ›
Chain: A
Length: 529 amino acids
Theoretical weight: 60.58 KDa
Source organism: Clostridium perfringens ATCC 13124
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2YW34 (Residues: 715-1220; Coverage: 24%)
Gene name: CPF_1073
Sequence domains: Peptidase M60, enhancin and enhancin-like

Ligands and Environments

2 bound ligands:
Ligand MES 2 x MES
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 60.168Å b: 54.553Å c: 90.984Å
α: 90° β: 93.2° γ: 90°
R-values:
R R work R free
0.191 0.189 0.228
Expression system: Escherichia coli BL21(DE3)

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