6xw7

X-ray diffraction
2.15Å resolution

Crystal structure of murine norovirus P domain in complex with Nanobody NB-5829 and glycochenodeoxycholate (GCDCA)

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-212522 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Capsid protein VP1 Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 309 amino acids
Theoretical weight: 33.68 KDa
Source organism: Murine norovirus 1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q80J94 (Residues: 226-533; Coverage: 57%)
Gene name: ORF2
Sequence domains: Calicivirus coat protein C-terminal
Nanobody NB-5829 Chains: C, D, G, H
Molecule details ›
Chains: C, D, G, H
Length: 123 amino acids
Theoretical weight: 13.45 KDa
Source organism: Vicugna pacos
Expression system: Escherichia coli

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: C2
Unit cell:
a: 190.73Å b: 124.93Å c: 113.13Å
α: 90° β: 125.3° γ: 90°
R-values:
R R work R free
0.174 0.171 0.215
Expression systems:
  • Escherichia coli BL21
  • Escherichia coli