› 6y2u

X-ray diffraction
1.93Å resolution

Crystal structure of the cAMP-dependent protein kinase A cocrystallized with aminofasudil and PKI (5-24)

Released:
DOI: 10.2210/pdb6y2u/pdb
Model geometry
Fit model/data
PDB entry 6y2u coloured by chain, front view.
PDB entry 6y2u coloured by chain, side view.
PDB entry 6y2u coloured by chain, top view.
Source organisms:
Entry authors: Oebbeke M, Gerber H-D, Heine A, Klebe G

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150426 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
cAMP-dependent protein kinase catalytic subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 353 amino acids
Theoretical weight: 41.11 KDa
Source organism: Cricetulus griseus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25321 (Residues: 1-351; Coverage: 100%)
Gene name: PRKACA
Sequence domains: Protein kinase domain
cAMP-dependent protein kinase inhibitor alpha Chain: B
Molecule details ›
Chain: B
Length: 20 amino acids
Theoretical weight: 2.23 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P63248 (Residues: 6-25; Coverage: 26%)
Gene name: Pkia

Ligands and Environments

1 bound ligand:
Ligand LRT 1 x LRT
2 modified residues:
Ligand SEP 2 x SEP
Ligand TPO 1 x TPO

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 58.706Å b: 71.119Å c: 107.976Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.222
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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