6y40

X-ray diffraction
1.75Å resolution

14-3-3 Sigma in complex with phosphorylated PLN peptide

Released:
Source organism: Homo sapiens
Primary publication:
A new soaking procedure for X-ray crystallographic structural determination of protein-peptide complexes.
OpenAccess logo Acta Crystallogr F Struct Biol Commun 76 501-507 (2020)
PMID: 33006579

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-150829 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Phospholamban Chain: P
Molecule details ›
Chain: P
Length: 16 amino acids
Theoretical weight: 1.95 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P26678 (Residues: 6-21; Coverage: 31%)
Gene names: PLB, PLN
14-3-3 protein sigma Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 28.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P31947 (Residues: 1-248; Coverage: 100%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein

Ligands and Environments

2 bound ligands:
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2221
Unit cell:
a: 82.3Å b: 112.39Å c: 62.569Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.207
Expression systems:
  • Not provided
  • Escherichia coli