6y79

Electron Microscopy
2.96Å resolution

Cryo-EM structure of a respiratory complex I F89A mutant

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 42-mer (preferred)
PDBe Complex ID:
PDB-CPX-103387 (preferred)
Entry contents:
42 distinct polypeptide molecules
Macromolecules (42 distinct):
NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial Chain: B
Molecule details ›
Chain: B
Length: 488 amino acids
Theoretical weight: 53.83 KDa
Source organism: Yarrowia lipolytica
UniProt:
  • Canonical: F2Z660 (Residues: 1-488; Coverage: 100%)
Gene name: YALI0_B20372g
Sequence domains:
NADH-quinone oxidoreductase subunit D domain-containing protein Chain: C
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 Chain: D
NAD-dependent epimerase/dehydratase domain-containing protein Chain: E
ETC complex I subunit conserved region-domain-containing protein Chain: F
NADH:ubiquinone oxidoreductase 30kDa subunit domain-containing protein Chain: G
YALI0D00737p Chain: H
4Fe-4S ferredoxin-type domain-containing protein Chain: I
YALI0E11891p Chain: J
NADH:ubiquinone oxidoreductase-like 20kDa subunit domain-containing protein Chain: K
NADH-ubiquinone oxidoreductase chain 4L Chain: L
Zinc finger CHCC-type domain-containing protein Chain: M
Acyl carrier protein Chain: O
Complex 1 LYR protein domain-containing protein Chain: P
Acyl carrier protein Chain: Q
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 Chain: R
NUWM protein Chain: S
NADH-ubiquinone oxidoreductase Chain: U
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 Chain: W
NADH-ubiquinone oxidoreductase 21 kDa subunit Chain: X
NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial Chain: Y
NADH-ubiquinone oxidoreductase 21.3 kDa subunit Chain: Z
YALI0D04939p Chain: a
Subunit NEBM of NADH:Ubiquinone Oxidoreductase (Complex I) Chain: b
NADH-ubiquinone oxidoreductase B12 subunit family-domain-containing protein Chain: c
NADH-ubiquinone oxidoreductase 12 kDa subunit Chain: d
Subunit NUVM of NADH:Ubiquinone Oxidoreductase (Complex I) Chain: e
Ribosomal protein/NADH dehydrogenase domain-containing protein Chain: f
NADH-ubiquinone oxidoreductase 9.5 kDa subunit Chain: g
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit N7BM Chain: h
Subunit NUUM of NADH:Ubiquinone Oxidoreductase (Complex I) Chain: i
NADH-ubiquinone oxidoreductase B15 subunit Chain: j
YALI0F14003p Chain: n
NADH-ubiquinone oxidoreductase chain 1 Chain: 1
NADH-ubiquinone oxidoreductase chain 2 Chain: 2
NADH-ubiquinone oxidoreductase chain 3 Chain: 3
NADH-ubiquinone oxidoreductase chain 4 Chain: 4
NADH-ubiquinone oxidoreductase chain 5 Chain: 5
NADH-ubiquinone oxidoreductase chain 6 Chain: 6
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 Chain: 8
NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 Chain: 9

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 2.96Å
Relevant EMDB volumes: EMD-10711