› 6y7u

X-ray diffraction
1.6Å resolution

Structure of Chloroflexus aggregans Cagg_3753 LOV domain C85A A95P variant (CagFbFP)

Released:
DOI: 10.2210/pdb6y7u/pdb
PDB entry 6y7u coloured by chain, front view.
PDB entry 6y7u coloured by chain, side view.
PDB entry 6y7u coloured by chain, top view.
Source organism: Chloroflexus aggregans DSM 9485
Primary publication:
Effects of Proline Substitutions on the Thermostable LOV Domain from Chloroflexus aggregans
Remeeva A, Nazarenko VV, Goncharov IM, Yudenko A, Smolentseva A, Semenov O, Kovalev K, Gulbahar C, Schwaneberg U, Davari MD, Gordeliy V, Gushchin I
Crystals (2020)

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110712 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 113 amino acids
Theoretical weight: 12.43 KDa
Source organism: Chloroflexus aggregans DSM 9485
Expression system: Escherichia coli
UniProt:
  • Canonical: B8GAY9 (Residues: 47-153; Coverage: 12%)
Gene name: Cagg_3753
Sequence domains: PAS domain

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX IV BEAMLINE BioMAX
Spacegroup: P21212
Unit cell:
a: 54.256Å b: 111.36Å c: 38.935Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.178 0.225
Expression system: Escherichia coli

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