Function and Biology Details
Reactions catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.
Peptidylproline (omega=180) = peptidylproline (omega=0)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- Retrovirus capsid, N-terminal
- Retroviral nucleocapsid Gag protein p24, C-terminal domain
- Retrovirus capsid, C-terminal
- Retroviral nucleocapsid Gag protein p24, N-terminal
- Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain
- Cyclophilin-like domain superfamily
- Cyclophilin-type peptidyl-prolyl cis-trans isomerase, conserved site
- Cyclophilin-type peptidyl-prolyl cis-trans isomerase
Structure analysis Details
Assembly composition:
hetero tridecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138040 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Capsid protein p24
Molecule details ›
Chains: A, B, C, D, G, H, N, Y, d, e, j, k
Length: 220 amino acids
Theoretical weight: 24.53 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 220 amino acids
Theoretical weight: 24.53 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli
UniProt:
- Canonical:
P04591 (Residues: 133-352; Coverage: 44%)
Sequence domains:
Peptidyl-prolyl cis-trans isomerase A
Molecule details ›
Chain: J
Length: 164 amino acids
Theoretical weight: 17.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
Length: 164 amino acids
Theoretical weight: 17.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical: P62937 (Residues: 2-165; Coverage: 99%)
Sequence domains: Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
