6yam

Electron Microscopy
3.6Å resolution

Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA

Released:
Related structures: EMD-10761

Function and Biology Details

Reaction catalysed:
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 50-mer (preferred)
PDBe Complex ID:
PDB-CPX-278905 (preferred)
Entry contents:
47 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (50 distinct):
Small ribosomal subunit protein eS32 Chain: l
Molecule details ›
Chain: l
Length: 25 amino acids
Theoretical weight: 3.47 KDa
Source organism: Oryctolagus cuniculus
Small ribosomal subunit protein uS2 Chain: C
Molecule details ›
Chain: C
Length: 208 amino acids
Theoretical weight: 23.36 KDa
Source organism: Oryctolagus cuniculus
Small ribosomal subunit protein eS1 Chain: D
40S ribosomal protein uS5 Chain: E
Small ribosomal subunit protein uS3 Chain: F
Small ribosomal subunit protein eS4 Chain: G
Small ribosomal subunit protein uS7 Chain: H
Small ribosomal subunit protein eS6 Chain: I
Small ribosomal subunit protein eS7 Chain: J
Small ribosomal subunit protein eS8 Chain: K
Small ribosomal subunit protein uS4 Chain: L
Small ribosomal subunit protein eS10 Chain: M
Small ribosomal subunit protein uS17 Chain: N
Small ribosomal subunit protein eS12 Chain: O
Small ribosomal subunit protein uS15 Chain: P
Small ribosomal subunit protein uS11 Chain: Q
Small ribosomal subunit protein uS9 Chain: S
Small ribosomal subunit protein eS17 Chain: T
Small ribosomal subunit protein eS19 Chain: V
Small ribosomal subunit protein uS10 Chain: W
Small ribosomal subunit protein eS21 Chain: X
Small ribosomal subunit protein uS8 Chain: Y
Small ribosomal subunit protein uS12 Chain: Z
40S ribosomal protein S24 Chain: a
Small ribosomal subunit protein eS26 Chain: b
Small ribosomal subunit protein eS27 Chain: c
Small ribosomal subunit protein eS28 Chain: d
Small ribosomal subunit protein uS14 Chain: e
Small ribosomal subunit protein eS31 Chain: f
Small ribosomal subunit protein RACK1 Chain: g
Small ribosomal subunit protein eS25 Chain: n
Small ribosomal subunit protein eS30 Chain: i
Eukaryotic translation initiation factor 2 subunit 1 Chain: A
eukaryotic translation initiation factor 2 subunit gamma Chain: B
eukaryotic translation initiation factor 1A Chain: j
ATP-binding cassette sub-family E member 1 (ABCE1) Chain: k
Small ribosomal subunit protein uS13 Chain: U
Small ribosomal subunit protein uS19 Chain: R
eukaryotic translation initiation factor 3 subunit d Chain: m
Eukaryotic translation initiation factor 3 subunit A Chain: y
Eukaryotic translation initiation factor 3 subunit C Chain: v
Eukaryotic translation initiation factor 3 subunit E Chain: w
Eukaryotic translation initiation factor 3 subunit F Chain: q
eukaryotic translation initiation factor 3 subunit h Chain: r
Eukaryotic translation initiation factor 3 subunit K Chain: s
eukaryotic translation initiation factor 3 subunit l Chain: t
Eukaryotic translation initiation factor 3 subunit M Chain: u
initiator methionylated tRNA Chain: 1
18S Ribosomal RNA Chain: 2
beta-globin mRNA Chain: 3

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.6Å
Relevant EMDB volumes: EMD-10761
Expression system: Not provided