› 6yif

X-ray diffraction
1.81Å resolution

Structure of Chromosomal Passenger Complex (CPC) bound to phosphorylated Histone 3 peptide at 1.8 A.

Released:
DOI: 10.2210/pdb6yif/pdb
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PDB entry 6yif coloured by chain, front view.
PDB entry 6yif coloured by chain, side view.
PDB entry 6yif coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis of MKLP2-dependent Aurora B transport from chromatin to the anaphase central spindle.
Serena M, Bastos RN, Elliott PR, Barr FA
OpenAccess logo J Cell Biol 219 (2020)
PMID: 32356865

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-127457 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Baculoviral IAP repeat-containing protein 5 Chain: A
Molecule details ›
Chain: A
Length: 144 amino acids
Theoretical weight: 16.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15392 (Residues: 1-142; Coverage: 100%)
Gene names: API4, BIRC5, IAP4
Sequence domains: Inhibitor of Apoptosis domain
Borealin Chain: B
Molecule details ›
Chain: B
Length: 67 amino acids
Theoretical weight: 8.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53HL2 (Residues: 10-76; Coverage: 24%)
Gene names: CDCA8, PESCRG3
Sequence domains: Nbl1 / Borealin N terminal
Inner centromere protein Chain: C
Histone H3.1 Chain: D

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand SO4 3 x SO4
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P65
Unit cell:
a: 98.863Å b: 98.863Å c: 56.067Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.181 0.223
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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Citations

2 review citations

The Abscission Checkpoint: A Guardian of Chromosomal Stability.
Petsalaki et al. (2021)
1 more

1 mention without citation

Molecular basis of MKLP2-dependent Aurora B transport from chromatin to the anaphase central spindle.
Serena et al. (2020)

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