› 6yoe

X-ray diffraction
1.85Å resolution

Structure of Lysozyme from SiN IMISX setup collected by still serial crystallography on crystals prelocated by 2D X-ray phase-contrast imaging

Released:
DOI: 10.2210/pdb6yoe/pdb
PDB entry 6yoe coloured by chain, front view.
PDB entry 6yoe coloured by chain, side view.
PDB entry 6yoe coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Low-dose in situ prelocation of protein microcrystals by 2D X-ray phase-contrast imaging for serial crystallography.
Martiel I, Huang CY, Villanueva-Perez P, Panepucci E, Basu S, Caffrey M, Pedrini B, Bunk O, Stampanoni M, Wang M
OpenAccess logo IUCrJ 7 1131-1141 (2020)
PMID: 33209324

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
Expression system: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family

Ligands and Environments

4 bound ligands:
Ligand AE3 1 x AE3
Ligand BR 7 x BR
Ligand NA 1 x NA
Ligand ACY 1 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P43212
Unit cell:
a: 78.5Å b: 78.5Å c: 40.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.264 0.263 0.285
Expression system: Gallus gallus

Quick links

Citations

1 citations in other articles

Application of Nanometer Heavy-duty Coating in the Optimization of Process Parameters for Power Generation Machinery.
Long W. (2022)

1 mention without citation

Low-dose in situ prelocation of protein microcrystals by 2D X-ray phase-contrast imaging for serial crystallography.
Martiel et al. (2020)