6ypu › Citations

Structure of the 70S Ribosome from the Human Pathogen Acinetobacter baumannii in Complex with Clinically Relevant Antibiotics.
<div class='caption-title'>Structure of the 70S Ribosome from A. baumannii</div>
<div class='caption-title'>Unique Structural Features of the A. baumannii Ribosome</div>
Nicholson D, Edwards TA, O'Neill AJ, Ranson NA
OpenAccess logo Structure 28 1087-1100.e3 (2020)
Related entries: 6yhs, 6ys5, 6ysi, 6yt9, 6ytf

Cited: 8 times
EuropePMC logo PMID: 32857965

Abstract

Acinetobacter baumannii is a Gram-negative bacterium primarily associated with hospital-acquired, often multidrug-resistant (MDR) infections. The ribosome-targeting antibiotics amikacin and tigecycline are among the limited arsenal of drugs available for treatment of such infections. We present high-resolution structures of the 70S ribosome from A. baumannii in complex with these antibiotics, as determined by cryoelectron microscopy. Comparison with the ribosomes of other bacteria reveals several unique structural features at functionally important sites, including around the exit of the polypeptide tunnel and the periphery of the subunit interface. The structures also reveal the mode and site of interaction of these drugs with the ribosome. This work paves the way for the design of new inhibitors of translation to address infections caused by MDR A. baumannii.

Reviews - 6ypu mentioned but not cited (1)

  1. Ribosome-targeting antibiotics and resistance via ribosomal RNA methylation. Jeremia L, Deprez BE, Dey D, Conn GL, Wuest WM. RSC Med Chem 14 624-643 (2023)

Articles - 6ypu mentioned but not cited (2)

  1. Structure of the 70S Ribosome from the Human Pathogen Acinetobacter baumannii in Complex with Clinically Relevant Antibiotics. Nicholson D, Edwards TA, O'Neill AJ, Ranson NA. Structure 28 1087-1100.e3 (2020)
  2. Mechanistic insights into translation inhibition by aminoglycoside antibiotic arbekacin. Parajuli NP, Mandava CS, Pavlov MY, Sanyal S. Nucleic Acids Res 49 6880-6892 (2021)


Articles citing this publication (5)

  1. Cryo-EM Determination of Eravacycline-Bound Structures of the Ribosome and the Multidrug Efflux Pump AdeJ of Acinetobacter baumannii. Zhang Z, Morgan CE, Bonomo RA, Yu EW. mBio 12 e0103121 (2021)
  2. A large expert-curated cryo-EM image dataset for machine learning protein particle picking. Dhakal A, Gyawali R, Wang L, Cheng J. Sci Data 10 392 (2023)
  3. Sarecycline inhibits protein translation in Cutibacterium acnes 70S ribosome using a two-site mechanism. Lomakin IB, Devarkar SC, Patel S, Grada A, Bunick CG. Nucleic Acids Res 51 2915-2930 (2023)
  4. Molecular basis of the pleiotropic effects by the antibiotic amikacin on the ribosome. Seely SM, Parajuli NP, De Tarafder A, Ge X, Sanyal S, Gagnon MG. Nat Commun 14 4666 (2023)
  5. Structural conservation of antibiotic interaction with ribosomes. Paternoga H, Crowe-McAuliffe C, Bock LV, Koller TO, Morici M, Beckert B, Myasnikov AG, Grubmüller H, Nováček J, Wilson DN. Nat Struct Mol Biol 30 1380-1392 (2023)


Quick links