› 6yt4

X-ray diffraction
2.4Å resolution

Crystal structure of the N112A mutant of the light-driven sodium pump KR2 in the pentameric form, pH 8.0

Released:
DOI: 10.2210/pdb6yt4/pdb
PDB entry 6yt4 coloured by chain, front view.
PDB entry 6yt4 coloured by chain, side view.
PDB entry 6yt4 coloured by chain, top view.
Source organism: Dokdonia eikasta
Primary publication:
Crystal Structure of the N112A Mutant of the Light-Driven Sodium Pump KR2
Maliar N, Kovalev K, Baeken C, Balandin T, Astashkin R, Rulev M, Alekseev A, Ilyinsky N, Rogachev A, Chupin V, Dolgikh D, Kirpichnikov M, Gordeliy V
Crystals (2020)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126095 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sodium pumping rhodopsin Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 288 amino acids
Theoretical weight: 32.57 KDa
Source organism: Dokdonia eikasta
Expression system: Escherichia coli
UniProt:
  • Canonical: N0DKS8 (Residues: 1-280; Coverage: 100%)
Gene name: NaR
Sequence domains: Bacteriorhodopsin-like protein

Ligands and Environments

6 bound ligands:
Ligand OLC 5 x OLC
Ligand OLA 16 x OLA
Ligand LFA 28 x LFA
Ligand BOG 5 x BOG
Ligand RET 5 x RET
Ligand NA 5 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2221
Unit cell:
a: 129.84Å b: 239.7Å c: 134.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.217
Expression system: Escherichia coli

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