› 6ytw

X-ray diffraction
2Å resolution

CLK3 bound with benzothiazole Tg003 (Cpd 2)

Released:
DOI: 10.2210/pdb6ytw/pdb
PDB entry 6ytw coloured by chain, front view.
PDB entry 6ytw coloured by chain, side view.
PDB entry 6ytw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
DFG-1 Residue Controls Inhibitor Binding Mode and Affinity, Providing a Basis for Rational Design of Kinase Inhibitor Selectivity.
Schröder M, Bullock AN, Fedorov O, Bracher F, Chaikuad A, Knapp S
J Med Chem 63 10224-10234 (2020)
PMID: 32787076

Function and Biology Details

Reaction catalysed: 
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L-seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156013 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dual specificity protein kinase CLK3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 360 amino acids
Theoretical weight: 42.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49761 (Residues: 127-484; Coverage: 73%)
Gene name: CLK3
Sequence domains: Protein kinase domain

Ligands and Environments

5 bound ligands:
Ligand EDO 18 x EDO
Ligand CL 1 x CL
Ligand PO4 2 x PO4
Ligand EAE 2 x EAE
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: C2
Unit cell:
a: 96.057Å b: 131.599Å c: 83.598Å
α: 90° β: 107.98° γ: 90°
R-values:
R R work R free
0.186 0.184 0.227
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Cdc2-like kinases: structure, biological function, and therapeutic targets for diseases.
Song et al. (2023)
8 other articles

2 mentions without citation

Dual-Specificity, Tyrosine Phosphorylation-Regulated Kinases (DYRKs) and cdc2-Like Kinases (CLKs) in Human Disease, an Overview.
Lindberg et al. (2021)
1 more