› 6yw6

Electron Microscopy
4.2Å resolution

Cryo-EM structure of the ARP2/3 1B5CL isoform complex.

Released:
DOI: 10.2210/pdb6yw6/pdb
PDB entry 6yw6 coloured by chain, front view.
PDB entry 6yw6 coloured by chain, side view.
PDB entry 6yw6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM of human Arp2/3 complexes provides structural insights into actin nucleation modulation by ARPC5 isoforms.
von Loeffelholz O, Purkiss A, Cao L, Kjaer S, Kogata N, Romet-Lemonne G, Way M, Moores CA
OpenAccess logo Biol Open 9 (2020)
PMID: 32661131
Related structures: EMD-10959

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127309 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Actin-related protein 3 Chain: A
Molecule details ›
Chain: A
Length: 418 amino acids
Theoretical weight: 47.43 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P61158 (Residues: 1-418; Coverage: 100%)
Gene names: ACTR3, ARP3
Sequence domains: Actin
Actin-related protein 2/3 complex subunit 1B Chain: C
Molecule details ›
Chain: C
Length: 372 amino acids
Theoretical weight: 41 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O15143 (Residues: 1-372; Coverage: 100%)
Gene names: ARC41, ARPC1B
Sequence domains: WD domain, G-beta repeat
Actin-related protein 2/3 complex subunit 2 Chain: D
Molecule details ›
Chain: D
Length: 300 amino acids
Theoretical weight: 34.39 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O15144 (Residues: 1-300; Coverage: 100%)
Gene names: ARC34, ARPC2, PRO2446
Sequence domains: Arp2/3 complex, 34 kD subunit p34-Arc
Actin-related protein 2/3 complex subunit 3 Chain: E
Molecule details ›
Chain: E
Length: 178 amino acids
Theoretical weight: 20.57 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O15145 (Residues: 1-178; Coverage: 100%)
Gene names: ARC21, ARPC3
Sequence domains: ARP2/3 complex ARPC3 (21 kDa) subunit
Actin-related protein 2 Chain: B
Molecule details ›
Chain: B
Length: 394 amino acids
Theoretical weight: 44.82 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P61160 (Residues: 1-394; Coverage: 100%)
Gene names: ACTR2, ARP2
Sequence domains: Actin
Actin-related protein 2/3 complex subunit 5-like protein Chain: G
Molecule details ›
Chain: G
Length: 153 amino acids
Theoretical weight: 16.96 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9BPX5 (Residues: 1-153; Coverage: 100%)
Gene name: ARPC5L
Sequence domains: ARP2/3 complex 16 kDa subunit (p16-Arc)
Actin-related protein 2/3 complex subunit 4 Chain: F
Molecule details ›
Chain: F
Length: 168 amino acids
Theoretical weight: 19.7 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59998 (Residues: 1-168; Coverage: 100%)
Gene names: ARC20, ARPC4
Sequence domains: ARP2/3 complex 20 kDa subunit (ARPC4)

Ligands and Environments

1 bound ligand:
Ligand ATP 2 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-10959
Expression system: Spodoptera frugiperda

Quick links

Citations

2 review citations

Nucleation, stabilization, and disassembly of branched actin networks.
Gautreau et al. (2022)
1 more

3 mentions without citation

Is Your Kid Actin Out? A Series of Six Patients With Inherited Actin-Related Protein 2/3 Complex Subunit 1B Deficiency and Review of the Literature.
Vásquez-Echeverri et al. (2023)
2 more