6z0q

X-ray diffraction
1.54Å resolution

Crystal structure of SAICAR Synthetase (PurC) from Mycobacterium abscessus in complex with fragment 2

Released:

Function and Biology Details

Reaction catalysed:
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-109355 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylaminoimidazole-succinocarboxamide synthase Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 33.9 KDa
Source organism: Mycobacteroides abscessus ATCC 19977
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B1MHW4 (Residues: 1-297; Coverage: 100%)
Gene names: MAB_0689, purC
Sequence domains: SAICAR synthetase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P21
Unit cell:
a: 48.247Å b: 65.201Å c: 49.051Å
α: 90° β: 110.73° γ: 90°
R-values:
R R work R free
0.189 0.187 0.231
Expression system: Escherichia coli BL21(DE3)