› 6z1e

X-ray diffraction
2.45Å resolution

Crystal structure of the AAA domain of Rubisco Activase from Nostoc sp. (strain PCC 7120)

Released:
DOI: 10.2210/pdb6z1e/pdb
PDB entry 6z1e coloured by chain, front view.
PDB entry 6z1e coloured by chain, side view.
PDB entry 6z1e coloured by chain, top view.
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Primary publication:
Dual Functions of a Rubisco Activase in Metabolic Repair and Recruitment to Carboxysomes.
Flecken M, Wang H, Popilka L, Hartl FU, Bracher A, Hayer-Hartl M
Cell 183 457-473.e20 (2020)
PMID: 32979320

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157704 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribulose bisphosphate carboxylase/oxygenase activase Chains: A, B
Molecule details ›
Chains: A, B
Length: 290 amino acids
Theoretical weight: 32.77 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P58555 (Residues: 2-291; Coverage: 70%)
Gene names: alr1533, rca
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand CL 3 x CL
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: R32
Unit cell:
a: 112.92Å b: 112.92Å c: 284.327Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.212 0.264
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

New discoveries expand possibilities for carboxysome engineering.
Borden et al. (2021)
4 more