› 6z3s

X-ray diffraction
3.05Å resolution

Crystal structure of delta466-491 cystathionine beta-synthase from Toxoplasma gondii with O-Acetylserine

Released:
DOI: 10.2210/pdb6z3s/pdb
PDB entry 6z3s coloured by chain, front view.
PDB entry 6z3s coloured by chain, side view.
PDB entry 6z3s coloured by chain, top view.
Source organism: Toxoplasma gondii ME49
Primary publication:
Structural insight into the unique conformation of cystathionine β-synthase from Toxoplasma gondii.
Fernández-Rodríguez C, Oyenarte I, Conter C, González-Recio I, Núñez-Franco R, Gil-Pitarch C, Quintana I, Jiménez-Osés G, Dominici P, Martinez-Chantar ML, Astegno A, Martínez-Cruz LA
Comput Struct Biotechnol J 19 3542-3555 (2021)
PMID: 34194677

Function and Biology Details

Reaction catalysed: 
L-serine + L-homocysteine = L-cystathionine + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102882 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystathionine beta-synthase Chains: A, B, D
Molecule details ›
Chains: A, B, D
Length: 490 amino acids
Theoretical weight: 53.38 KDa
Source organism: Toxoplasma gondii ME49
Expression system: Toxoplasma gondii ME49
UniProt:
  • Canonical: A0A125YSJ9 (Residues: 1-514; Coverage: 95%)
Gene name: TGME49_259180
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand P1T 3 x P1T
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P3121
Unit cell:
a: 82.7Å b: 82.7Å c: 422.968Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.269 0.268 0.288
Expression system: Toxoplasma gondii ME49

Quick links

Citations

3 citation in other articles

H2S biogenesis by cystathionine beta-synthase: mechanism of inhibition by aminooxyacetic acid and unexpected role of serine.
Petrosino et al. (2022)
2 more