› 6z4a

X-ray diffraction
1.46Å resolution

Structure of the human SAS-6 N-terminal domain, F131E mutant

Released:
DOI: 10.2210/pdb6z4a/pdb
PDB entry 6z4a coloured by chain, front view.
PDB entry 6z4a coloured by chain, side view.
PDB entry 6z4a coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Identification of compounds that bind the centriolar protein SAS-6 and inhibit its oligomerization.
Busch JMC, Matsoukas MT, Musgaard M, Spyroulias GA, Biggin PC, Vakonakis I
OpenAccess logo J Biol Chem 295 17922-17934 (2020)
PMID: 32873708

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180415 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spindle assembly abnormal protein 6 homolog Chain: A
Molecule details ›
Chain: A
Length: 154 amino acids
Theoretical weight: 17.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6UVJ0 (Residues: 1-152; Coverage: 23%)
Gene names: SAS6, SASS6
Sequence domains: Centriolar protein SAS N-terminal domain

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21212
Unit cell:
a: 59.59Å b: 65.13Å c: 38.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly.
Hatzopoulos et al. (2021)

1 mention without citation

Identification of compounds that bind the centriolar protein SAS-6 and inhibit its oligomerization.
Busch et al. (2020)