› 6z7w

X-ray diffraction
2.42Å resolution

Human insulin in complex with the analytical antibody HUI-018 Fab

Released:
DOI: 10.2210/pdb6z7w/pdb
PDB entry 6z7w coloured by chain, front view.
PDB entry 6z7w coloured by chain, side view.
PDB entry 6z7w coloured by chain, top view.
Source organisms:
Primary publication:
Insulin binding to the analytical antibody sandwich pair OXI-005 and HUI-018: Epitope mapping and binding properties.
Johansson E, Wu X, Yu B, Yang Z, Cao Z, Wiberg C, Jeppesen CB, Poulsen F
Protein Sci 30 485-496 (2021)
PMID: 33277949

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-208400 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
MAb 6H10 light chain Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 214 amino acids
Theoretical weight: 23.81 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
HUI-018 Fab Heavy Chain Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 224 amino acids
Theoretical weight: 23.72 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Insulin A chain Chains: I, K, M, O
Molecule details ›
Chains: I, K, M, O
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chains: J, L, N, P
Molecule details ›
Chains: J, L, N, P
Length: 30 amino acids
Theoretical weight: 3.43 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P21
Unit cell:
a: 50.23Å b: 216.61Å c: 101.09Å
α: 90° β: 103.84° γ: 90°
R-values:
R R work R free
0.211 0.209 0.275
Expression systems:
  • Homo sapiens
  • Saccharomyces cerevisiae

Quick links

Citations

1 citations in other articles

Islet Autoantibody Standardization Program: interlaboratory comparison of insulin autoantibody assay performance in 2018 and 2020 workshops.
Marzinotto et al. (2023)

1 mention without citation

Insulin binding to the analytical antibody sandwich pair OXI-005 and HUI-018: Epitope mapping and binding properties.
Johansson et al. (2021)