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Steps toward translocation-independent RNA polymerase inactivation by terminator ATPase ρ.

Said N, Hilal T, Sunday ND, Khatri A, Bürger J, Mielke T, Belogurov GA, Loll B, Sen R, Artsimovitch I, Wahl MC
Science 371 (2021)
Related entries: 6z9p, 6z9r, 6z9s, 6z9t, 7adb, 7adc, 7add, 7ade

Cited: 46 times
EuropePMC logo PMID: 33243850

Abstract

Factor-dependent transcription termination mechanisms are poorly understood. We determined a series of cryo-electron microscopy structures portraying the hexameric adenosine triphosphatase (ATPase) ρ on a pathway to terminating NusA/NusG-modified elongation complexes. An open ρ ring contacts NusA, NusG, and multiple regions of RNA polymerase, trapping and locally unwinding proximal upstream DNA. NusA wedges into the ρ ring, initially sequestering RNA. Upon deflection of distal upstream DNA over the RNA polymerase zinc-binding domain, NusA rotates underneath one capping ρ subunit, which subsequently captures RNA. After detachment of NusG and clamp opening, RNA polymerase loses its grip on the RNA:DNA hybrid and is inactivated. Our structural and functional analyses suggest that ρ, and other termination factors across life, may use analogous strategies to allosterically trap transcription complexes in a moribund state.

Articles - 6z9q mentioned but not cited (1)

  1. Steps toward translocation-independent RNA polymerase inactivation by terminator ATPase ρ. Said N, Hilal T, Sunday ND, Khatri A, Bürger J, Mielke T, Belogurov GA, Loll B, Sen R, Artsimovitch I, Wahl MC. Science 371 eabd1673 (2021)


Reviews citing this publication (13)

  1. Mechanisms of hexameric helicases. Fernandez AJ, Berger JM. Crit Rev Biochem Mol Biol 56 621-639 (2021)
  2. The many faces of RNA-based RNase P, an RNA-world relic. Phan HD, Lai LB, Zahurancik WJ, Gopalan V. Trends Biochem Sci 46 976-991 (2021)
  3. Bacterial transcription during growth arrest. Bergkessel M. Transcription 12 232-249 (2021)
  4. RNA polymerases from low G+C gram-positive bacteria. Miller M, Oakley AJ, Lewis PJ. Transcription 12 92-102 (2021)
  5. Rho-dependent transcription termination: a revisionist view. Hao Z, Svetlov V, Nudler E. Transcription 12 171-181 (2021)
  6. Composition of Transcription Machinery and Its Crosstalk with Nucleoid-Associated Proteins and Global Transcription Factors. Muskhelishvili G, Sobetzko P, Mehandziska S, Travers A. Biomolecules 11 924 (2021)
  7. Macromolecular assemblies supporting transcription-translation coupling. Webster MW, Weixlbaumer A. Transcription 12 103-125 (2021)
  8. Structural advances in transcription elongation. Mohamed AA, Vazquez Nunez R, Vos SM. Curr Opin Struct Biol 75 102422 (2022)
  9. β-CASP proteins removing RNA polymerase from DNA: when a torpedo is needed to shoot a sitting duck. Wiedermannová J, Krásný L. Nucleic Acids Res 49 10221-10234 (2021)
  10. Coupled Transcription-Translation in Prokaryotes: An Old Couple With New Surprises. Irastortza-Olaziregi M, Amster-Choder O. Front Microbiol 11 624830 (2020)
  11. Factor-stimulated intrinsic termination: getting by with a little help from some friends. Mandell ZF, Zemba D, Babitzke P. Transcription 13 96-108 (2022)
  12. NusG, an Ancient Yet Rapidly Evolving Transcription Factor. Wang B, Artsimovitch I. Front Microbiol 11 619618 (2020)
  13. Suppressor Mutants: History and Today's Applications. Bautista DE, Carr JF, Mitchell AM. EcoSal Plus 9 eESP00372020 (2021)

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