› 6zej

X-ray diffraction
1.78Å resolution

Structure of PP1-Phactr1 chimera [PP1(7-304) + linker (SGSGS) + Phactr1(526-580)]

Released:
DOI: 10.2210/pdb6zej/pdb
PDB entry 6zej coloured by chain, front view.
PDB entry 6zej coloured by chain, side view.
PDB entry 6zej coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for substrate specificity of the Phactr1/PP1 phosphatase holoenzyme.
Fedoryshchak RO, Přechová M, Butler AM, Lee R, O'Reilly N, Flynn HR, Snijders AP, Eder N, Ultanir S, Mouilleron S, Treisman R
OpenAccess logo Elife 9 (2020)
PMID: 32975518

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-238752 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatase and actin regulator 1; Serine/threonine-protein phosphatase PP1-alpha catalytic subunit Chains: A, D, F, I, L, O
Molecule details ›
Chains: A, D, F, I, L, O
Length: 363 amino acids
Theoretical weight: 41.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62136 (Residues: 7-304; Coverage: 90%)
  • Canonical: Q9C0D0 (Residues: 526-580; Coverage: 10%)
Gene names: KIAA1733, PHACTR1, PPP1A, PPP1CA, RPEL1
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand MN 12 x MN
Ligand GOL 7 x GOL
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P65
Unit cell:
a: 137.37Å b: 137.37Å c: 238.02Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.243 0.242 0.271
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Regulation of Synaptic Transmission and Plasticity by Protein Phosphatase 1.
Foley et al. (2021)
2 more

1 mention without citation

Molecular basis for substrate specificity of the Phactr1/PP1 phosphatase holoenzyme.
Fedoryshchak et al. (2020)