› 6zet

Electron crystallography
2.7Å resolution

Crystal structure of proteinase K nanocrystals by electron diffraction with a 20 micrometre C2 condenser aperture

Released:
DOI: 10.2210/pdb6zet/pdb
PDB entry 6zet coloured by chain, front view.
PDB entry 6zet coloured by chain, side view.
PDB entry 6zet coloured by chain, top view.
Source organism: Parengyodontium album
Primary publication:
A Workflow for Protein Structure Determination From Thin Crystal Lamella by Micro-Electron Diffraction.
Beale EV, Waterman DG, Hecksel C, van Rooyen J, Gilchrist JB, Parkhurst JM, de Haas F, Buijsse B, Evans G, Zhang P
OpenAccess logo Front Mol Biosci 7 179 (2020)
PMID: 32850967

Function and Biology Details

Reaction catalysed: 
Hydrolysis of keratin, and of other proteins with subtilisin-like specificity. Hydrolyzes peptide amides.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139275 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proteinase K Chain: AAA
Molecule details ›
Chain: AAA
Length: 279 amino acids
Theoretical weight: 28.96 KDa
Source organism: Parengyodontium album
Expression system: Not provided
UniProt:
  • Canonical: P06873 (Residues: 106-384; Coverage: 76%)
Gene name: PROK
Sequence domains: Subtilase family

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.701Å
Expression system: Not provided

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Citations

3 review citations

3D Electron Diffraction for Chemical Analysis: Instrumentation Developments and Innovative Applications.
Gruene et al. (2021)
2 more

3 mentions without citation

Electron Diffraction of 3D Molecular Crystals.
Saha et al. (2022)
2 more