› 6zfg

X-ray diffraction
1.85Å resolution

14-3-3 zeta chimera with 18E6 and fusicoccin

Released:
DOI: 10.2210/pdb6zfg/pdb
PDB entry 6zfg coloured by chain, front view.
PDB entry 6zfg coloured by chain, side view.
PDB entry 6zfg coloured by chain, top view.
Source organisms:
Primary publication:
Hierarchized phosphotarget binding by the seven human 14-3-3 isoforms.
Gogl G, Tugaeva KV, Eberling P, Kostmann C, Trave G, Sluchanko NN
OpenAccess logo Nat Commun 12 1677 (2021)
PMID: 33723253

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139050 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
14-3-3 protein zeta/delta; Protein E6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 243 amino acids
Theoretical weight: 27.39 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P63104 (Residues: 1-229; Coverage: 94%)
  • Canonical: P06463 (Residues: 152-158; Coverage: 4%)
Gene names: E6, YWHAZ
Sequence domains: 14-3-3 protein

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand FSC 2 x FSC
1 modified residue:
Ligand TPO 2 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 73.23Å b: 76.1Å c: 88.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.219
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Endocytosis and Transcytosis of SARS-CoV-2 Across the Intestinal Epithelium and Other Tissue Barriers.
Knyazev et al. (2021)
9 more

2 mentions without citation

Hierarchized phosphotarget binding by the seven human 14-3-3 isoforms.
Gogl et al. (2021)
1 more