› 6zi5

X-ray diffraction
2.8Å resolution

Ultrafast Structural Response to Charge Redistribution Within a Photosynthetic Reaction Centre - 300 ps (a) structure

Released:
DOI: 10.2210/pdb6zi5/pdb
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PDB entry 6zi5 coloured by chain, front view.
PDB entry 6zi5 coloured by chain, side view.
PDB entry 6zi5 coloured by chain, top view.
Source organism: Blastochloris viridis
Primary publication:
Ultrafast structural changes within a photosynthetic reaction centre.
Dods R, Båth P, Morozov D, Gagnér VA, Arnlund D, Luk HL, Kübel J, Maj M, Vallejos A, Wickstrand C, Bosman R, Beyerlein KR, Nelson G, Liang M, Milathianaki D, Robinson J, Harimoorthy R, Berntsen P, Malmerberg E, Johansson L, Andersson R, Carbajo S, Claesson E, Conrad CE, Dahl P, Hammarin G, Hunter MS, Li C, Lisova S, Royant A, Safari C, Sharma A, Williams GJ, Yefanov O, Westenhoff S, Davidsson J, DePonte DP, Boutet S, Barty A, Katona G, Groenhof G, Brändén G, Neutze R
Nature 589 310-314 (2021)
PMID: 33268896

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-138851 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Photosynthetic reaction center cytochrome c subunit Chain: C
Molecule details ›
Chain: C
Length: 336 amino acids
Theoretical weight: 37.45 KDa
Source organism: Blastochloris viridis
UniProt:
  • Canonical: P07173 (Residues: 21-356; Coverage: 100%)
Gene names: BVIRIDIS_00500, cytC, pufC
Sequence domains: Photosynthetic reaction centre cytochrome C subunit
Reaction center protein H chain Chain: H
Molecule details ›
Chain: H
Length: 258 amino acids
Theoretical weight: 28.56 KDa
Source organism: Blastochloris viridis
UniProt:
  • Canonical: P06008 (Residues: 1-258; Coverage: 100%)
Gene name: puhA
Sequence domains:
Reaction center protein L chain Chain: L
Reaction center protein M chain Chain: M

Ligands and Environments

10 bound ligands:
Ligand HEC 4 x HEC
Ligand DGA 1 x DGA
Ligand SO4 12 x SO4
Ligand LDA 4 x LDA
Ligand HTO 2 x HTO
Ligand BCB 4 x BCB
Ligand BPB 2 x BPB
Ligand FE 1 x FE
Ligand MQ7 1 x MQ7
Ligand NS5 1 x NS5
1 modified residue:
Ligand FME 1 x FME

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLAC LCLS BEAMLINE CXI
Spacegroup: P43212
Unit cell:
a: 226.4Å b: 226.4Å c: 113.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.202

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Citations

6 review citations

Fake It 'Till You Make It-The Pursuit of Suitable Membrane Mimetics for Membrane Protein Biophysics.
Thoma et al. (2020)
5 more

2 mentions without citation

Absence of electron-transfer-associated changes in the time-dependent X-ray free-electron laser structures of the photosynthetic reaction center.
Nishikawa et al. (2023)
1 more

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