› 6znj

X-ray diffraction
3.7Å resolution

MaeB full-length enzyme apoprotein form

Released:
DOI: 10.2210/pdb6znj/pdb
PDB entry 6znj coloured by chain, front view.
PDB entry 6znj coloured by chain, side view.
PDB entry 6znj coloured by chain, top view.
Source organism: Bdellovibrio bacteriovorus HD100
Primary publication:
A rotary mechanism for allostery in bacterial hybrid malic enzymes.
Harding CJ, Cadby IT, Moynihan PJ, Lovering AL
Nat Commun 12 1228 (2021)
PMID: 33623032

Function and Biology Details

Reaction catalysed: 
(S)-malate + NADP(+) = pyruvate + CO(2) + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179894 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malic enzyme N-terminal domain-containing protein; Malic enzyme NAD-binding domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 791 amino acids
Theoretical weight: 85.95 KDa
Source organism: Bdellovibrio bacteriovorus HD100
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6MM14 (Residues: 1-120; Coverage: 98%)
  • Canonical: Q6MM15 (Residues: 2-657; Coverage: 100%)
Gene names: Bd1833, Bd1834, mdh
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2221
Unit cell:
a: 81.705Å b: 273.583Å c: 308.232Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.27
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Bdellovibrio bacteriovorus phosphoglucose isomerase structures reveal novel rigidity in the active site of a selected subset of enzymes upon substrate binding.
Meek et al. (2021)
2 more