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Structure of plant photosystem I-plastocyanin complex reveals strong hydrophobic interactions.

<div class='caption-title'>Structure of pea Photosystem I in-complex with reduced pea Pc and analysis of the solvent inaccessible region formed by their bound interfaces.</div>
<div class='caption-title'>In-silico model of Pc dissociation from PSI.</div>
<div class='caption-title'>PsaA residues Arg647 and Asp648 are required for an efficient binding of the electron donor Pc.</div>
Caspy I, Fadeeva M, Kuhlgert S, Borovikova-Sheinker A, Klaiman D, Masrati G, Drepper F, Ben-Tal N, Hippler M, Nelson N
OpenAccess logo Biochem J 478 2371-2384 (2021)
Cited: 8 times
EuropePMC logo PMID: 34085703

Abstract

Photosystem I is defined as plastocyanin-ferredoxin oxidoreductase. Taking advantage of genetic engineering, kinetic analyses and cryo-EM, our data provide novel mechanistic insights into binding and electron transfer between PSI and Pc. Structural data at 2.74 Å resolution reveals strong hydrophobic interactions in the plant PSI-Pc ternary complex, leading to exclusion of water molecules from PsaA-PsaB/Pc interface once the PSI-Pc complex forms. Upon oxidation of Pc, a slight tilt of bound oxidized Pc allows water molecules to accommodate the space between Pc and PSI to drive Pc dissociation. Such a scenario is consistent with the six times larger dissociation constant of oxidized as compared with reduced Pc and mechanistically explains how this molecular machine optimized electron transfer for fast turnover.

Articles - 6zoo mentioned but not cited (3)

  1. Structure of plant photosystem I-plastocyanin complex reveals strong hydrophobic interactions. Caspy I, Fadeeva M, Kuhlgert S, Borovikova-Sheinker A, Klaiman D, Masrati G, Drepper F, Ben-Tal N, Hippler M, Nelson N. Biochem J 478 2371-2384 (2021)
  2. Algal photosystem I dimer and high-resolution model of PSI-plastocyanin complex. Naschberger A, Mosebach L, Tobiasson V, Kuhlgert S, Scholz M, Perez-Boerema A, Ho TTH, Vidal-Meireles A, Takahashi Y, Hippler M, Amunts A. Nat Plants 8 1191-1201 (2022)
  3. Structure of cyanobacterial photosystem I complexed with ferredoxin at 1.97 Å resolution. Li J, Hamaoka N, Makino F, Kawamoto A, Lin Y, Rögner M, Nowaczyk MM, Lee YH, Namba K, Gerle C, Kurisu G. Commun Biol 5 951 (2022)


Reviews citing this publication (1)

  1. Photosystem I Inhibition, Protection and Signalling: Knowns and Unknowns. Lima-Melo Y, Kılıç M, Aro EM, Gollan PJ. Front Plant Sci 12 791124 (2021)

Articles citing this publication (4)

  1. Dimeric and high-resolution structures of Chlamydomonas Photosystem I from a temperature-sensitive Photosystem II mutant. Caspy I, Schwartz T, Bayro-Kaiser V, Fadeeva M, Kessel A, Ben-Tal N, Nelson N. Commun Biol 4 1380 (2021)
  2. Flavodiiron-mediated O2 photoreduction at photosystem I acceptor-side provides photoprotection to conifer thylakoids in early spring. Bag P, Shutova T, Shevela D, Lihavainen J, Nanda S, Ivanov AG, Messinger J, Jansson S. Nat Commun 14 3210 (2023)
  3. Structure of Photosystem I Supercomplex Isolated from a Chlamydomonas reinhardtii Cytochrome b6f Temperature-Sensitive Mutant. Schwartz T, Fadeeva M, Klaiman D, Nelson N. Biomolecules 13 537 (2023)
  4. Structure of the Acidobacteria homodimeric reaction center bound with cytochrome c. Dong S, Huang G, Wang C, Wang J, Sui SF, Qin X. Nat Commun 13 7745 (2022)


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