› 6zsn

X-ray diffraction
2.6Å resolution

Crystal structure of rsGCaMP double mutant Ile80His/Val116Ile in the OFF state (illuminated)

Released:
DOI: 10.2210/pdb6zsn/pdb
PDB entry 6zsn coloured by chain, front view.
PDB entry 6zsn coloured by chain, side view.
PDB entry 6zsn coloured by chain, top view.
Source organisms:
Primary publication:
Genetically encoded photo-switchable molecular sensors for optoacoustic and super-resolution imaging.
Mishra K, Fuenzalida-Werner JP, Pennacchietti F, Janowski R, Chmyrov A, Huang Y, Zakian C, Klemm U, Testa I, Niessing D, Ntziachristos V, Stiel AC
OpenAccess logo Nat Biotechnol 40 598-605 (2022)
PMID: 34845372

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125520 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
EF-hand domain-containing protein; Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 418 amino acids
Theoretical weight: 47.32 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: K4DIE3 (Residues: 51-59, 60-94, 95-127, 128-147, 218-218, 225-269, 295-300, 312-336, 348-362, 369-432; Coverage: 56%)
  • Canonical: P42212 (Residues: 2-78, 80-87, 133-159, 166-176, 183-193, 209-214; Coverage: 56%)
Gene name: GFP
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand FMT 6 x FMT
Ligand CA 4 x CA
Ligand NA 1 x NA
1 modified residue:
Ligand PIA 1 x PIA

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P41212
Unit cell:
a: 118.97Å b: 118.97Å c: 96.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.191 0.259
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Looking deep inside tissue with photoacoustic molecular probes: a review.
Hui et al. (2022)
1 more