6ztm

Electron Microscopy
3.3Å resolution

E. coli 70S-RNAP expressome complex in collided state without NusG

Released:
Source organism: Escherichia coli
Related structures: EMD-11420

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 60-mer (preferred)
PDBe Complex ID:
PDB-CPX-199530 (preferred)
Entry contents:
51 distinct polypeptide molecules
2 distinct DNA molecules
6 distinct RNA molecules
Macromolecules (59 distinct):
Small ribosomal subunit protein uS2 Chain: AB
Molecule details ›
Chain: AB
Length: 241 amino acids
Theoretical weight: 26.78 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7V0 (Residues: 1-241; Coverage: 100%)
Gene names: JW0164, b0169, rpsB
Sequence domains: Ribosomal protein S2
Small ribosomal subunit protein uS3 Chain: AC
Molecule details ›
Chain: AC
Length: 233 amino acids
Theoretical weight: 26.03 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7V3 (Residues: 1-233; Coverage: 100%)
Gene names: JW3276, b3314, rpsC
Sequence domains:
Small ribosomal subunit protein uS4 Chain: AD
Small ribosomal subunit protein uS5 Chain: AE
Small ribosomal subunit protein bS6 Chain: AF
Small ribosomal subunit protein uS7 Chain: AG
Small ribosomal subunit protein uS8 Chain: AH
Small ribosomal subunit protein uS9 Chain: AI
Small ribosomal subunit protein uS10 Chain: AJ
Small ribosomal subunit protein uS11 Chain: AK
Small ribosomal subunit protein uS12 Chain: AL
Small ribosomal subunit protein uS13 Chain: AM
Small ribosomal subunit protein uS14 Chain: AN
Small ribosomal subunit protein uS15 Chain: AO
Small ribosomal subunit protein bS16 Chain: AP
Small ribosomal subunit protein uS17 Chain: AQ
Small ribosomal subunit protein bS18 Chain: AR
Small ribosomal subunit protein uS19 Chain: AS
Small ribosomal subunit protein bS20 Chain: AT
Small ribosomal subunit protein bS21 Chain: AU
Large ribosomal subunit protein uL2 Chain: BC
Large ribosomal subunit protein uL3 Chain: BD
Large ribosomal subunit protein uL4 Chain: BE
Large ribosomal subunit protein uL5 Chain: BF
Large ribosomal subunit protein uL6 Chain: BG
Large ribosomal subunit protein bL9 Chain: BH
Large ribosomal subunit protein uL13 Chain: BK
Large ribosomal subunit protein uL14 Chain: BL
Large ribosomal subunit protein uL15 Chain: BM
Large ribosomal subunit protein uL16 Chain: BN
Large ribosomal subunit protein bL17 Chain: BO
Large ribosomal subunit protein uL18 Chain: BP
Large ribosomal subunit protein bL19 Chain: BQ
Large ribosomal subunit protein bL20 Chain: BR
Large ribosomal subunit protein bL21 Chain: BS
Large ribosomal subunit protein uL22 Chain: BT
Large ribosomal subunit protein uL23 Chain: BU
Large ribosomal subunit protein uL24 Chain: BV
Large ribosomal subunit protein bL25 Chain: BW
Large ribosomal subunit protein bL27 Chain: BX
Large ribosomal subunit protein bL28 Chain: BY
Large ribosomal subunit protein uL29 Chain: BZ
Large ribosomal subunit protein uL30 Chain: B1
Large ribosomal subunit protein bL32 Chain: B2
Large ribosomal subunit protein bL33 Chain: B3
Large ribosomal subunit protein bL34 Chain: B4
Large ribosomal subunit protein bL35 Chain: B5
Large ribosomal subunit protein bL36A Chain: B6
DNA-directed RNA polymerase subunit alpha Chains: CA, CB
DNA-directed RNA polymerase subunit beta Chain: CC
DNA-directed RNA polymerase subunit beta' Chain: CD
Non-template DNA strand Chain: CN
Template DNA strand Chain: CT
16S ribosomal RNA Chain: AA
Messenger RNA Chain: AV
tRNA(fmet) P-site Chain: AW
Phe-NH-tRNA(Phe) A-site Chain: AX
23S ribosomal RNA Chain: BA
5S ribosomal RNA Chain: BB

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-11420
Expression system: Escherichia coli