› 6zu6

X-ray diffraction
1.15Å resolution

Cu nitrite reductase from Achromobacter cycloclastes: MSOX series at 170K, dose point 1

Released:
DOI: 10.2210/pdb6zu6/pdb
PDB entry 6zu6 coloured by chain, front view.
PDB entry 6zu6 coloured by chain, side view.
PDB entry 6zu6 coloured by chain, top view.
Source organism: Achromobacter cycloclastes
Primary publication:
Nature of the copper-nitrosyl intermediates of copper nitrite reductases during catalysis.
Hough MA, Conradie J, Strange RW, Antonyuk SV, Eady RR, Ghosh A, Hasnain SS
OpenAccess logo Chem Sci 11 12485-12492 (2020)
PMID: 34094452

Function and Biology Details

Reaction catalysed: 
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-540824 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper-containing nitrite reductase Chain: A
Molecule details ›
Chain: A
Length: 334 amino acids
Theoretical weight: 36.62 KDa
Source organism: Achromobacter cycloclastes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25006 (Residues: 45-378; Coverage: 98%)
Gene name: nirK
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand CU 2 x CU
Ligand NO2 2 x NO2
Ligand SO4 2 x SO4
Ligand MLI 1 x MLI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P213
Unit cell:
a: 95.34Å b: 95.34Å c: 95.34Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.12 0.118 0.159
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Single crystal spectroscopy and multiple structures from one crystal (MSOX) define catalysis in copper nitrite reductases.
Rose et al. (2022)