› 6zy9

Electron Microscopy
3.3Å resolution

Cryo-EM structure of MlaFEDB in complex with AMP-PNP

Released:
DOI: 10.2210/pdb6zy9/pdb
PDB entry 6zy9 coloured by chain, front view.
PDB entry 6zy9 coloured by chain, side view.
PDB entry 6zy9 coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into outer membrane asymmetry maintenance in Gram-negative bacteria by MlaFEDB.
Tang X, Chang S, Qiao W, Luo Q, Chen Y, Jia Z, Coleman J, Zhang K, Wang T, Zhang Z, Zhang C, Zhu X, Wei X, Dong C, Zhang X, Dong H
Nat Struct Mol Biol 28 81-91 (2021)
PMID: 33199922
Related structures: EMD-11555

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-245106 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Intermembrane phospholipid transport system binding protein MlaD Chains: A, D, I, J, K, L
Molecule details ›
Chains: A, D, I, J, K, L
Length: 183 amino acids
Theoretical weight: 19.59 KDa
Source organism: Escherichia coli B185
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P64604 (Residues: 1-183; Coverage: 100%)
Gene names: JW3160, b3193, mlaD, yrbD
Sequence domains: MlaD protein
Intermembrane phospholipid transport system binding protein MlaB Chains: B, C
Molecule details ›
Chains: B, C
Length: 105 amino acids
Theoretical weight: 11.8 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P64602 (Residues: 1-97; Coverage: 100%)
Gene names: JW5535, b3191, mlaB, yrbB
Sequence domains: STAS domain
Toluene tolerance protein Ttg2A Chains: F, G
Molecule details ›
Chains: F, G
Length: 269 amino acids
Theoretical weight: 29.13 KDa
Source organism: Escherichia coli 909945-2
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
Uncharacterized protein Chains: E, H
Molecule details ›
Chains: E, H
Length: 260 amino acids
Theoretical weight: 27.89 KDa
Source organism: Escherichia coli 2.3916
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Ligands and Environments

2 bound ligands:
Ligand ANP 2 x ANP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-11555
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

6 review citations

Structural and functional diversity calls for a new classification of ABC transporters.
Thomas et al. (2020)
5 more

2 mentions without citation

Structure and mechanism of the bacterial lipid ABC transporter, MlaFEDB.
Ekiert et al. (2022)
1 more