› 6zys

X-ray diffraction
1.87Å resolution

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156597 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase IMP-1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 230 amino acids
Theoretical weight: 25.3 KDa
Source organism: Serratia marcescens
Expression system: Escherichia coli
UniProt:
  • Canonical: P52699 (Residues: 19-246; Coverage: 100%)
Sequence domains: Metallo-beta-lactamase superfamily

Ligands and Environments

3 bound ligands:
Ligand ZN 8 x ZN
Ligand QST 4 x QST
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 48.782Å b: 77.969Å c: 261.906Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.174 0.201
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Metallo-β-lactamases in the Age of Multidrug Resistance: From Structure and Mechanism to Evolution, Dissemination, and Inhibitor Design.
Bahr et al. (2021)
4 more

1 mention without citation

A multiscale approach to predict the binding mode of metallo beta-lactamase inhibitors.
Gervasoni et al. (2022)