› 6zyw

Electron Microscopy
8.78Å resolution

Outer Dynein Arm-Shulin complex - overall structure (Tetrahymena thermophila)

Released:
DOI: 10.2210/pdb6zyw/pdb
PDB entry 6zyw coloured by chain, front view.
PDB entry 6zyw coloured by chain, side view.
PDB entry 6zyw coloured by chain, top view.
Source organism: Tetrahymena thermophila SB210
Primary publication:
Shulin packages axonemal outer dynein arms for ciliary targeting.
Mali GR, Ali FA, Lau CK, Begum F, Boulanger J, Howe JD, Chen ZA, Rappsilber J, Skehel M, Carter AP
Science 371 910-916 (2021)
PMID: 33632841
Related structures: EMD-11576

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonadecamer (preferred)
PDBe Complex ID:
PDB-CPX-107447 (preferred)
Entry contents:
17 distinct polypeptide molecules
Macromolecules (17 distinct):
Dynein-1-alpha heavy chain, flagellar inner arm I1 complex protein, putative Chain: A
Molecule details ›
Chain: A
Length: 4168 amino acids
Theoretical weight: 475.55 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:
  • Canonical: I7M6H4 (Residues: 1-4168; Coverage: 100%)
Gene name: TTHERM_00486600
Sequence domains:
Outer arm dynein beta heavy chain Chain: B
Molecule details ›
Chain: B
Length: 4595 amino acids
Theoretical weight: 530.18 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:
  • Canonical: I7M9J2 (Residues: 1-4595; Coverage: 100%)
Gene name: TTHERM_00499300
Sequence domains:
AAA+ ATPase domain-containing protein Chain: C
Molecule details ›
Chain: C
Length: 4620 amino acids
Theoretical weight: 534.33 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:
  • Canonical: Q22A67 (Residues: 1-4620; Coverage: 100%)
Gene name: TTHERM_01276420
Sequence domains:
Dynein intermediate chain Chains: D, d
Molecule details ›
Chains: D, d
Length: 667 amino acids
Theoretical weight: 77.89 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:
  • Canonical: I7M008 (Residues: 1-667; Coverage: 100%)
Gene name: TTHERM_00487150
Sequence domains: WD domain, G-beta repeat
Flagellar outer dynein arm intermediate protein, putative Chains: E, e
Molecule details ›
Chains: E, e
Length: 670 amino acids
Theoretical weight: 77.18 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:
  • Canonical: Q23FU1 (Residues: 1-670; Coverage: 100%)
Gene name: TTHERM_00079230
Roadblock/LAMTOR2 domain-containing protein Chain: F
Molecule details ›
Chain: F
Length: 133 amino acids
Theoretical weight: 14.75 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:
  • Canonical: I7MHB1 (Residues: 1-133; Coverage: 100%)
Gene name: TTHERM_00348650
Sequence domains: Roadblock/LC7 domain
Roadblock/LAMTOR2 domain-containing protein Chain: G
Molecule details ›
Chain: G
Length: 103 amino acids
Theoretical weight: 11.63 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:
  • Canonical: Q22MV2 (Residues: 57-159; Coverage: 65%)
Gene name: TTHERM_00030210
Sequence domains: Roadblock/LC7 domain
Dynein light chain Chain: H
Molecule details ›
Chain: H
Length: 92 amino acids
Theoretical weight: 10.78 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:
  • Canonical: A4VE64 (Residues: 1-92; Coverage: 100%)
Gene name: TTHERM_00449029
Sequence domains: Dynein light chain type 1
Dynein light chain Chain: I
Molecule details ›
Chain: I
Length: 110 amino acids
Theoretical weight: 12.35 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:
  • Canonical: I7MCM8 (Residues: 1-110; Coverage: 100%)
Gene name: TTHERM_00716250
Sequence domains: Dynein light chain type 1
Dynein light chain Chain: J
Molecule details ›
Chain: J
Length: 93 amino acids
Theoretical weight: 10.97 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:
  • Canonical: Q24DI9 (Residues: 1-93; Coverage: 100%)
Gene name: TTHERM_00971840
Sequence domains: Dynein light chain type 1
Dynein light chain Chain: K
Molecule details ›
Chain: K
Length: 111 amino acids
Theoretical weight: 13.34 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:
  • Canonical: Q22R86 (Residues: 1-111; Coverage: 100%)
Gene name: TTHERM_00023950
Sequence domains: Dynein light chain type 1
Dynein light chain Chain: L
Molecule details ›
Chain: L
Length: 111 amino acids
Theoretical weight: 12.52 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:
  • Canonical: W7XJB1 (Residues: 1-111; Coverage: 100%)
Gene name: TTHERM_000442909
Sequence domains: Dynein light chain type 1
Dynein light chain Chain: M
Molecule details ›
Chain: M
Length: 87 amino acids
Theoretical weight: 10.45 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:
  • Canonical: Q24CE5 (Residues: 1-87; Coverage: 100%)
Gene name: TTHERM_01079060
Sequence domains: Dynein light chain type 1
Dynein light chain 2A Chain: N
Molecule details ›
Chain: N
Length: 132 amino acids
Theoretical weight: 15.61 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:
  • Canonical: Q1HGH8 (Residues: 1-132; Coverage: 100%)
Sequence domains: Tctex-1 family
Dynein light chain tctex-type 1 protein Chain: O
Molecule details ›
Chain: O
Length: 117 amino acids
Theoretical weight: 13.2 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:
  • Canonical: A4VEB3 (Residues: 1-117; Coverage: 100%)
Gene name: TTHERM_00392979
Sequence domains: Tctex-1 family
Thioredoxin Chain: P
Molecule details ›
Chain: P
Length: 110 amino acids
Theoretical weight: 12.86 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:
  • Canonical: Q240X9 (Residues: 1-110; Coverage: 100%)
Gene name: TTHERM_00623050
Sequence domains: Thioredoxin
Shulin Chain: Y
Molecule details ›
Chain: Y
Length: 1200 amino acids
Theoretical weight: 139.94 KDa
Source organism: Tetrahymena thermophila SB210
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q22YU3 (Residues: 1-1200; Coverage: 100%)
Gene name: TTHERM_00122270

Ligands and Environments

3 bound ligands:
Ligand ADP 3 x ADP
Ligand ATP 1 x ATP
Ligand GTP 1 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 8.78Å
Relevant EMDB volumes: EMD-11576
Expression system: Spodoptera frugiperda

Quick links

Citations

4 review citations

Structure and Mechanics of Dynein Motors.
Canty et al. (2021)
3 more

3 mentions without citation

Structure and Function of Dynein's Non-Catalytic Subunits.
Rao et al. (2024)
2 more