› 6zz8

X-ray diffraction
3.73Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108966 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Spindle assembly abnormal protein 6 N-terminal domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 227 amino acids
Theoretical weight: 25.7 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A9CQL4 (Residues: 2-226; Coverage: 31%)
Gene names: CHLRE_12g516950v5, CrSAS-6
Sequence domains:
Fibronectin type-III domain-containing protein Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 94 amino acids
Theoretical weight: 9.9 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: M1E1G6 (Residues: 7-90, 91-99; Coverage: 94%)
Sequence domains: Fibronectin type III domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P42212
Unit cell:
a: 257.16Å b: 257.16Å c: 125.289Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.225 0.267
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

2 review citations

Monobodies as tool biologics for accelerating target validation and druggable site discovery.
Akkapeddi et al. (2021)
1 more

1 mention without citation

Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly.
Hatzopoulos et al. (2021)