› 7a1h

X-ray diffraction
1.9Å resolution

Crystal structure of wild-type CI2

Released:
DOI: 10.2210/pdb7a1h/pdb
PDB entry 7a1h coloured by chain, front view.
PDB entry 7a1h coloured by chain, side view.
PDB entry 7a1h coloured by chain, top view.
Source organism: Hordeum vulgare
Primary publication:
Synergistic stabilization of a double mutant in chymotrypsin inhibitor 2 from a library screen in E. coli.
Hamborg L, Granata D, Olsen JG, Roche JV, Pedersen LE, Nielsen AT, Lindorff-Larsen K, Teilum K
OpenAccess logo Commun Biol 4 980 (2021)
PMID: 34408246

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133915 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Subtilisin-chymotrypsin inhibitor-2A Chain: A
Molecule details ›
Chain: A
Length: 64 amino acids
Theoretical weight: 7.31 KDa
Source organism: Hordeum vulgare
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P01053 (Residues: 21-84; Coverage: 76%)
Sequence domains: Potato inhibitor I family

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: Agilent SuperNova
Spacegroup: P622
Unit cell:
a: 68.266Å b: 68.266Å c: 52.605Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.203 0.254
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

4 citation in other articles

A dual-reporter system for investigating and optimizing protein translation and folding in E. coli.
Zutz et al. (2021)
3 more

2 mentions without citation

Synergistic stabilization of a double mutant in chymotrypsin inhibitor 2 from a library screen in E. coli.
Hamborg et al. (2021)
1 more