› 7aa2

X-ray diffraction
1.4Å resolution

Chaetomium thermophilum FAD-dependent oxidoreductase in complex with ABTS

Released:
DOI: 10.2210/pdb7aa2/pdb
PDB entry 7aa2 coloured by chain, front view.
PDB entry 7aa2 coloured by chain, side view.
PDB entry 7aa2 coloured by chain, top view.
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Primary publication:
Crystallographic fragment screening-based study of a novel FAD-dependent oxidoreductase from Chaetomium thermophilum.
Švecová L, Østergaard LH, Skálová T, Schnorr KM, Koval' T, Kolenko P, Stránský J, Sedlák D, Dušková J, Trundová M, Hašek J, Dohnálek J
OpenAccess logo Acta Crystallogr D Struct Biol 77 755-775 (2021)
PMID: 34076590

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124053 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
FAD-dependent oxidoreductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 595 amino acids
Theoretical weight: 64.27 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: G0SAW6 (Residues: 42-645; Coverage: 94%)
Gene name: CTHT_0048040
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
6 bound ligands:
Ligand FDA 2 x FDA
Ligand NAG 9 x NAG
Ligand EBS 2 x EBS
Ligand FMT 2 x FMT
Ligand MG 3 x MG
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P212121
Unit cell:
a: 93.589Å b: 109.799Å c: 115.982Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.129 0.128 0.172
Expression system: Aspergillus oryzae

Quick links

Citations

1 review citation

Glucose Oxidase, an Enzyme "Ferrari": Its Structure, Function, Production and Properties in the Light of Various Industrial and Biotechnological Applications.
Bauer et al. (2022)
4 other articles

2 mentions without citation

Crystallographic fragment screening-based study of a novel FAD-dependent oxidoreductase from Chaetomium thermophilum.
Švecová et al. (2021)
1 more