› 7aaw

X-ray diffraction
2.25Å resolution

Thioredoxin Reductase from Bacillus cereus

Released:
DOI: 10.2210/pdb7aaw/pdb
Model geometry
Fit model/data
PDB entry 7aaw coloured by chain, front view.
PDB entry 7aaw coloured by chain, side view.
PDB entry 7aaw coloured by chain, top view.
Source organism: Bacillus cereus ATCC 14579
Primary publication:
Thioredoxin reductase from Bacillus cereus exhibits distinct reduction and NADPH-binding properties.
Shoor M, Gudim I, Hersleth HP, Hammerstad M
OpenAccess logo FEBS Open Bio 11 3019-3031 (2021)
PMID: 34492167

Function and Biology Details

Reaction catalysed: 
Thioredoxin + NADP(+) = thioredoxin disulfide + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182434 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 321 amino acids
Theoretical weight: 35 KDa
Source organism: Bacillus cereus ATCC 14579
Expression system: Escherichia coli
UniProt:
  • Canonical: Q815J1 (Residues: 1-321; Coverage: 100%)
Gene name: BC_5159
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD

Cofactor: Ligand NAP 1 x NAP
3 bound ligands:
Ligand GLC 4 x GLC
Ligand ACT 1 x ACT
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P41212
Unit cell:
a: 94.98Å b: 94.98Å c: 214.622Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.178 0.219
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

A Fluorescent Probe to Detect Quick Disulfide Reductase Activity in Bacteria.
Zhao et al. (2022)
1 more

2 mentions without citation

Thioredoxin reductase from Bacillus cereus exhibits distinct reduction and NADPH-binding properties.
Shoor et al. (2021)
1 more

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO