› 7abw

X-ray diffraction
3.35Å resolution

Crystal structure of siderophore reductase FoxB

Released:
DOI: 10.2210/pdb7abw/pdb
PDB entry 7abw coloured by chain, front view.
PDB entry 7abw coloured by chain, side view.
PDB entry 7abw coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structural insights into a novel family of integral membrane siderophore reductases.
Josts I, Veith K, Normant V, Schalk IJ, Tidow H
Proc Natl Acad Sci U S A 118 (2021)
PMID: 34417315

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191258 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PepSY domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 382 amino acids
Theoretical weight: 43.11 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I117 (Residues: 1-382; Coverage: 100%)
Gene name: PA2465
Sequence domains: PepSY-associated TM region

Ligands and Environments

4 bound ligands:
Ligand HEM 4 x HEM
Ligand PE5 1 x PE5
Ligand DMU 5 x DMU
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: P21212
Unit cell:
a: 237.065Å b: 114.321Å c: 64.382Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.266 0.265 0.299
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Highlighting membrane protein structure and function: A celebration of the Protein Data Bank.
Li et al. (2021)
3 more

2 mentions without citation

Implications of AlphaFold2 for crystallographic phasing by molecular replacement.
McCoy et al. (2022)
1 more