› 7aon

X-ray diffraction
1.3Å resolution

Crystal structure of CI2 double mutant L49I,I57V

Released:
DOI: 10.2210/pdb7aon/pdb
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PDB entry 7aon coloured by chain, front view.
PDB entry 7aon coloured by chain, side view.
PDB entry 7aon coloured by chain, top view.
Source organism: Hordeum vulgare
Primary publication:
Synergistic stabilization of a double mutant in chymotrypsin inhibitor 2 from a library screen in E. coli.
Hamborg L, Granata D, Olsen JG, Roche JV, Pedersen LE, Nielsen AT, Lindorff-Larsen K, Teilum K
OpenAccess logo Commun Biol 4 980 (2021)
PMID: 34408246

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133915 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Subtilisin-chymotrypsin inhibitor-2A Chain: A
Molecule details ›
Chain: A
Length: 64 amino acids
Theoretical weight: 7.3 KDa
Source organism: Hordeum vulgare
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01053 (Residues: 21-84; Coverage: 76%)
Sequence domains: Potato inhibitor I family

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P622
Unit cell:
a: 68.392Å b: 68.392Å c: 52.661Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.165 0.162 0.219
Expression system: Escherichia coli BL21(DE3)

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Citations

5 citation in other articles

A dual-reporter system for investigating and optimizing protein translation and folding in E. coli.
Zutz et al. (2021)
4 more

1 mention without citation

Synergistic stabilization of a double mutant in chymotrypsin inhibitor 2 from a library screen in E. coli.
Hamborg et al. (2021)

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