7b2m

Electron Microscopy
3.39Å resolution

Cryo-EM structure of complement C4b in complex with nanobody E3

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-533391 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Complement C4 beta chain Chain: A
Molecule details ›
Chain: A
Length: 656 amino acids
Theoretical weight: 71.76 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P0C0L4 (Residues: 20-675; Coverage: 38%)
Gene names: C4A, CO4, CPAMD2
Sequence domains:
Complement C4-A alpha chain Chain: B
Molecule details ›
Chain: B
Length: 767 amino acids
Theoretical weight: 84.27 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P0C0L4 (Residues: 680-1446; Coverage: 45%)
Gene names: C4A, CO4, CPAMD2
Sequence domains:
Complement C4 gamma chain Chain: C
Molecule details ›
Chain: C
Length: 291 amino acids
Theoretical weight: 33.12 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P0C0L4 (Residues: 1454-1744; Coverage: 17%)
Gene names: C4A, CO4, CPAMD2
Sequence domains:
Anti-C4b nanobody E3 Chain: D
Molecule details ›
Chain: D
Length: 135 amino acids
Theoretical weight: 14.49 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.39Å
Relevant EMDB volumes: EMD-11988
Expression system: Escherichia coli BL21(DE3)