› 7b5q

Electron Microscopy
2.5Å resolution

Cryo-EM structure of the human CAK bound to ICEC0942 (PHENIX-OPLS3e)

Released:
DOI: 10.2210/pdb7b5q/pdb
PDB entry 7b5q coloured by chain, front view.
PDB entry 7b5q coloured by chain, side view.
PDB entry 7b5q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
2.5 Å-resolution structure of human CDK-activating kinase bound to the clinical inhibitor ICEC0942.
Greber BJ, Remis J, Ali S, Nogales E
OpenAccess logo Biophys J 120 677-686 (2021)
PMID: 33476598
Related structures: EMD-12042

Function and Biology Details

Reactions catalysed: 
ATP + a protein = ADP + a phosphoprotein
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156240 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
CDK-activating kinase assembly factor MAT1 Chain: H
Molecule details ›
Chain: H
Length: 328 amino acids
Theoretical weight: 38.13 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P51948 (Residues: 1-309; Coverage: 100%)
Gene names: CAP35, MAT1, MNAT1, RNF66
Sequence domains:
Cyclin-H Chain: I
Molecule details ›
Chain: I
Length: 323 amino acids
Theoretical weight: 37.7 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P51946 (Residues: 1-323; Coverage: 100%)
Gene name: CCNH
Sequence domains:
Cyclin-dependent kinase 7 Chain: J
Molecule details ›
Chain: J
Length: 391 amino acids
Theoretical weight: 43.65 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P50613 (Residues: 1-346; Coverage: 100%)
Gene names: CAK, CAK1, CDK7, CDKN7, MO15, STK1
Sequence domains: Protein kinase domain

Ligands and Environments

1 bound ligand:
Ligand I74 1 x I74
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.5Å
Relevant EMDB volumes: EMD-12042
Expression system: Trichoplusia ni

Quick links

Citations

2 review citations

Biophysical and Integrative Characterization of Protein Intrinsic Disorder as a Prime Target for Drug Discovery.
Luo et al. (2023)
1 more

1 mention without citation

2.5 Å-resolution structure of human CDK-activating kinase bound to the clinical inhibitor ICEC0942.
Greber et al. (2021)