› 7brq

X-ray diffraction
1.4Å resolution

Crystal structure of human FAM134B LIR fused to human GABARAP

Released:
DOI: 10.2210/pdb7brq/pdb
PDB entry 7brq coloured by chain, front view.
PDB entry 7brq coloured by chain, side view.
PDB entry 7brq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Super-assembly of ER-phagy receptor Atg40 induces local ER remodeling at contacts with forming autophagosomal membranes.
Mochida K, Yamasaki A, Matoba K, Kirisako H, Noda NN, Nakatogawa H
OpenAccess logo Nat Commun 11 3306 (2020)
PMID: 32620754

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131841 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gamma-aminobutyric acid receptor-associated protein; Reticulophagy regulator 1 Chain: A
Molecule details ›
Chain: A
Length: 137 amino acids
Theoretical weight: 16.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H6L5 (Residues: 450-468; Coverage: 4%)
  • Canonical: O95166 (Residues: 1-116; Coverage: 99%)
Gene names: FAM134B, FLC3B, GABARAP, HT004, JK1, RETREG1
Sequence domains: Autophagy protein Atg8 ubiquitin like

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: P63
Unit cell:
a: 81.892Å b: 81.892Å c: 33.324Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.192 0.206
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

ER-Phagy, ER Homeostasis, and ER Quality Control: Implications for Disease.
Ferro-Novick et al. (2021)
9 more

2 mentions without citation

Phosphorylation of FAM134C by CK2 controls starvation-induced ER-phagy.
Di Lorenzo et al. (2022)
1 more