› 7c38

X-ray diffraction
1.2Å resolution

Crystal structure of AofleA from Arthrobotrys oligospora in complex with L-fucose

Released:
DOI: 10.2210/pdb7c38/pdb
PDB entry 7c38 coloured by chain, front view.
PDB entry 7c38 coloured by chain, side view.
PDB entry 7c38 coloured by chain, top view.
Source organism: Orbilia oligospora ATCC 24927
Primary publication:
Structural insights into the fungi-nematodes interaction mediated by fucose-specific lectin AofleA from Arthrobotrys oligospora.
Liu M, Cheng X, Wang J, Tian D, Tang K, Xu T, Zhang M, Wang Y, Wang M
Int J Biol Macromol 164 783-793 (2020)
PMID: 32698064

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124241 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AofleA Chains: A, B
Molecule details ›
Chains: A, B
Length: 355 amino acids
Theoretical weight: 39.47 KDa
Source organism: Orbilia oligospora ATCC 24927
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: G1XA82 (Residues: 2-343; Coverage: 100%)
Gene name: AOL_s00076g540
Sequence domains: Fungal fucose-specific lectin

Ligands and Environments

3 bound ligands:
Ligand FUC 4 x FUC
Ligand FUL 4 x FUL
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 50.424Å b: 66.938Å c: 209.744Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.114 0.112 0.139
Expression system: Escherichia coli BL21

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Citations

1 review citation

Fungi-Nematode Interactions: Diversity, Ecology, and Biocontrol Prospects in Agriculture.
Zhang et al. (2020)
2 other articles