7c4p

X-ray diffraction
2Å resolution

Crystal structure of DBD plasma treated zebrafish TRF2 myb-domain complexed with DNA

Released:
Model geometry
Fit model/data
Source organism: Danio rerio
Entry authors: Jin Z, Park JH, Yun JH, Park SY, Lee W

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-121140 (preferred)
Entry contents:
2 distinct polypeptide molecules
3 distinct DNA molecules
Macromolecules (5 distinct):
Telomere repeat factor a Chain: A
Molecule details ›
Chain: A
Length: 55 amino acids
Theoretical weight: 6.68 KDa
Source organism: Danio rerio
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8JGS4 (Residues: 520-574; Coverage: 10%)
Gene name: terfa
Sequence domains: Myb-like DNA-binding domain
Terfa protein Chain: B
Molecule details ›
Chain: B
Length: 55 amino acids
Theoretical weight: 6.62 KDa
Source organism: Danio rerio
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4FZZ9 (Residues: 521-575; Coverage: 10%)
Gene name: terfa
Sequence domains: Myb-like DNA-binding domain
DNA (5'-D(*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*G)-3') Chain: C
DNA (5'-D(*CP*CP*CP*TP*AP*AP*CP*CP*CP*TP*AP*A)-3') Chains: D, F
DNA (5'-D(*TP*TP*AP*GP*GP*GP*TP*TP*AP*G)-3') Chain: E

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P3221
Unit cell:
a: 63.994Å b: 63.994Å c: 142.418Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.233 0.23 0.26
Expression system: Escherichia coli