› 7c6w

X-ray diffraction
1.88Å resolution

Crystal structure of beta-glycosides-binding protein (W177X) of ABC transporter in a closed state bound to laminaritetraose

Released:
DOI: 10.2210/pdb7c6w/pdb
PDB entry 7c6w coloured by chain, front view.
PDB entry 7c6w coloured by chain, side view.
PDB entry 7c6w coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Conformational Trapping of a β-Glucosides-Binding Protein Unveils the Selective Two-Step Ligand-Binding Mechanism of ABC Importers.
Chandravanshi M, Samanta R, Kanaujia SP
J Mol Biol 432 5711-5734 (2020)
PMID: 32866452

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176154 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Sugar ABC transporter, periplasmic sugar-binding protein Chain: A
Molecule details ›
Chain: A
Length: 422 amino acids
Theoretical weight: 45.91 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53W80 (Residues: 19-434; Coverage: 100%)
Gene name: TTHB082
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
5 bound ligands:
Ligand CL 1 x CL
Ligand SO2 1 x SO2
Ligand CO3 1 x CO3
Ligand EDO 2 x EDO
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 58.11Å b: 62.6Å c: 109.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.187 0.242
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Deciphering Cellodextrin and Glucose Uptake in Clostridium thermocellum.
Yan et al. (2022)
1 more