› 7dfb

X-ray diffraction
3.28Å resolution

Crystal of Arrestin2-V2Rpp-6-7-Fab30 complex

Released:
DOI: 10.2210/pdb7dfb/pdb
PDB entry 7dfb coloured by chain, front view.
PDB entry 7dfb coloured by chain, side view.
PDB entry 7dfb coloured by chain, top view.
Source organisms:
Primary publication:
Structural studies of phosphorylation-dependent interactions between the V2R receptor and arrestin-2.
He QT, Xiao P, Huang SM, Jia YL, Zhu ZL, Lin JY, Yang F, Tao XN, Zhao RJ, Gao FY, Niu XG, Xiao KH, Wang J, Jin C, Sun JP, Yu X
OpenAccess logo Nat Commun 12 2396 (2021)
PMID: 33888704

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-210716 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Beta-arrestin-1 Chain: A
Molecule details ›
Chain: A
Length: 426 amino acids
Theoretical weight: 48.27 KDa
Source organism: Bos taurus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P17870 (Residues: 1-418; Coverage: 100%)
Gene name: ARRB1
Sequence domains:
Vasopressin V2 receptor Chain: V
Molecule details ›
Chain: V
Length: 23 amino acids
Theoretical weight: 2.92 KDa
Source organism: synthetic construct
Expression system: Not provided
UniProt:
  • Canonical: P30518 (Residues: 346-368; Coverage: 6%)
Gene names: ADHR, AVPR2, DIR, DIR3, V2R
FAB30 LIGHT CHAIN Chain: L
Molecule details ›
Chain: L
Length: 227 amino acids
Theoretical weight: 24.75 KDa
Source organism: Mus musculus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
FAB30 HEAVY CHAIN Chain: H
Molecule details ›
Chain: H
Length: 249 amino acids
Theoretical weight: 26.83 KDa
Source organism: Mus musculus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Ligands and Environments

No bound ligands
2 modified residues:
Ligand TPO 3 x TPO
Ligand SEP 3 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17B1
Spacegroup: I212121
Unit cell:
a: 115.48Å b: 116.881Å c: 143.897Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.231 0.29
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided

Quick links

Citations

9 review citations

G protein-coupled receptor signaling: transducers and effectors.
Jiang et al. (2022)
8 more

1 mention without citation

Surveying nonvisual arrestins reveals allosteric interactions between functional sites.
Seckler et al. (2023)