› 7dq1

Electron Microscopy
3.6Å resolution

Cryo-EM structure of Coxsackievirus B1 virion in complex with CAR at physiological temperature

Released:
DOI: 10.2210/pdb7dq1/pdb
PDB entry 7dq1 coloured by chain, front view.
PDB entry 7dq1 coloured by chain, side view.
PDB entry 7dq1 coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structures reveal the molecular basis of receptor-initiated coxsackievirus uncoating.
Xu L, Zheng Q, Zhu R, Yin Z, Yu H, Lin Y, Wu Y, He M, Huang Y, Jiang Y, Sun H, Zha Z, Yang H, Huang Q, Zhang D, Chen Z, Ye X, Han J, Yang L, Liu C, Que Y, Fang M, Gu Y, Zhang J, Luo W, Zhou ZH, Li S, Cheng T, Xia N
Cell Host Microbe 29 448-462.e5 (2021)
PMID: 33539764
Related structures: EMD-30813

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 300-mer (preferred)
PDBe Complex ID:
PDB-CPX-496898 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Virion protein 1 Chain: 1
Molecule details ›
Chain: 1
Length: 278 amino acids
Theoretical weight: 31.21 KDa
Source organism: Coxsackievirus B1
UniProt:
  • Canonical: A0A7T7KAA0 (Residues: 571-848; Coverage: 33%)
Sequence domains: picornavirus capsid protein
VP2 Chain: 2
Molecule details ›
Chain: 2
Length: 263 amino acids
Theoretical weight: 29.12 KDa
Source organism: Coxsackievirus B1
UniProt:
  • Canonical: A0A2S0RQC2 (Residues: 70-332; Coverage: 12%)
Sequence domains: picornavirus capsid protein
VP3 Chain: 3
Molecule details ›
Chain: 3
Length: 238 amino acids
Theoretical weight: 26.33 KDa
Source organism: Coxsackievirus B1
UniProt:
  • Canonical: L7UV52 (Residues: 333-570; Coverage: 11%)
Sequence domains: picornavirus capsid protein
VP4 Chain: 4
Molecule details ›
Chain: 4
Length: 69 amino acids
Theoretical weight: 7.48 KDa
Source organism: Coxsackievirus B1
UniProt:
  • Canonical: A0A7T7KB31 (Residues: 1-69; Coverage: 100%)
Sequence domains: Picornavirus coat protein (VP4)
Coxsackievirus and adenovirus receptor Chain: K
Molecule details ›
Chain: K
Length: 121 amino acids
Theoretical weight: 13.47 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P78310 (Residues: 20-140; Coverage: 35%)
Gene names: CAR, CXADR
Sequence domains: Immunoglobulin V-set domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-30813

Quick links

Citations

2 review citations

Imaging Flow Cytometry and Confocal Immunofluorescence Microscopy of Virus-Host Cell Interactions.
McClelland et al. (2021)
1 more

1 mention without citation

Cryo-EM structures reveal the molecular basis of receptor-initiated coxsackievirus uncoating.
Xu et al. (2021)